OUR PREVIOUS GENERATION PROTOCOL IS DESCRIBED IN THE FOLLOWING PAPER:
www.ncbi.nlm.nih.gov/pubmed/18160042?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Res
ultsPanel.Pubmed_RVDocSum
Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis
transcriptome
Cell 2007 Dec 28;131(7):1340-53
Chekanova JA, Gregory BD, Reverdatto SV, Chen H, Kumar R, Hooker T, Yazaki J, Li P, Skiba N, Peng Q,
Alonso J, Brukhin V, Grossniklaus U, Ecker JR, Belostotsky DA
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA
The exosome complex plays a central and essential role in RNA metabolism. However, comprehensive
studies of exosome substrates and functional analyses of its subunits are lacking. Here, we demonstrate that
as opposed to yeast and metazoans the plant exosome core possesses an unanticipated functional plasticity
and present a genome-wide atlas of Arabidopsis exosome targets. Additionally, our study provides evidence
for widespread polyadenylation- and exosome-mediated RNA quality control in plants, reveals unexpected
aspects of stable structural RNA metabolism, and uncovers numerous novel exosome substrates. These
include a select subset of mRNAs, miRNA processing intermediates, and hundreds of noncoding RNAs, the
vast majority of which have not been previously described and belong to a layer of the transcriptome that can
only be visualized upon inhibition of exosome activity. These first genome-wide maps of exosome substrates
will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes
www.ncbi.nlm.nih.gov/pubmed/18160042?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Res
ultsPanel.Pubmed_RVDocSum
Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis
transcriptome
Cell 2007 Dec 28;131(7):1340-53
Chekanova JA, Gregory BD, Reverdatto SV, Chen H, Kumar R, Hooker T, Yazaki J, Li P, Skiba N, Peng Q,
Alonso J, Brukhin V, Grossniklaus U, Ecker JR, Belostotsky DA
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA
The exosome complex plays a central and essential role in RNA metabolism. However, comprehensive
studies of exosome substrates and functional analyses of its subunits are lacking. Here, we demonstrate that
as opposed to yeast and metazoans the plant exosome core possesses an unanticipated functional plasticity
and present a genome-wide atlas of Arabidopsis exosome targets. Additionally, our study provides evidence
for widespread polyadenylation- and exosome-mediated RNA quality control in plants, reveals unexpected
aspects of stable structural RNA metabolism, and uncovers numerous novel exosome substrates. These
include a select subset of mRNAs, miRNA processing intermediates, and hundreds of noncoding RNAs, the
vast majority of which have not been previously described and belong to a layer of the transcriptome that can
only be visualized upon inhibition of exosome activity. These first genome-wide maps of exosome substrates
will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes
Arabidopsis 2010 Program:
Supported by the National Science Foundation
Supported by the National Science Foundation
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